Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method) Clearance 2025 Unisex

Homocysteine (Hcy) Colorimetric Assay Kit (Enzyme Circulation Method)

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SKU # E-BC-K143 Detection Instrument Biochemistry analyzer (340 nm), Spectrophotometer (340 nm) Detection method Colorimetric method

Product Details

Properties

Synonyms	Hcy
Sample Type	Serum
Detection Range	0-50 μmol/L
Detection Method	Colorimetric method
Assay type	Quantitative
Storage	2-8℃
Valid period	12 months

Detection Principle

Oxidized homocysteine (HCY) is reduced to free homocysteine by triethyl phosphine (TCEP), and the free homocysteine reacts with substrate to generate adenosine. The generated adenosine is immediately dehydrogenated into inosine and ammonia, and the ammonia is further react with NADH under the catalysis of glutamate dehydrogenase to convert NADH to NAD+. The decrease in absorbance at 340 nm caused by the decline of NADH is proportional to the concentration of homocysteine in the sample.

 

Kit Components & Storage

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Item Component Specification Storage Reagent 1 S-adenosylmethionine 37 mL × 2 vials 2-8℃, 12 months, shading light NADH Tris (2-carboxyethyl) phosphonium chloride α-ketoglutaric acid Reagent 2 HCY methyltransferase 10 mL × 2 vials 2-8℃, 12 months, shading light Glutamate dehydrogenase S-adenosine homocysteine hydrolase Adenosine deaminase Mannitol Sodium azide Reagent 3 0 μmol/L Homocysteine Standard 1 mL × 1 vial 2-8℃, 12 months Reagent 4 28 μmol/L Homocysteine Standard 1 mL × 1 vial 2-8℃, 12 months

 

Note: The reagents must be stored strictly according to the preservation conditions in the above table. The reagents in different kits cannot be mixed with each other. For a small volume of reagents, please centrifuge before use, so as not to obtain sufficient amount of reagents.